Rate
Ask the Expert
Suggest an Application
Add to Favorites
CreateParts List
Tell a Friend
PrintFind methods for your needs
OSHA Method 41 for improved analysis of Diethylamine using the Thermo Scientific Acclaim Mixed-Mode HILIC-1 HPLC column
Description
As shown here, the Thermo Scientific Acclaim Mixed-Mode HILIC-1 can analyze the diethylamine derivative under the normal-phase conditions of OSHA Method 41. In this case, 4-chloro-7-nitro-1,2,3-benzoxadiazole (NBD-chloride) reacts with amines in the trap, to produce a highly-colored, fluorescent derivative. The unreacted reagent and a trace of monoethylamine eluted at 2.3 and 4.4 minutes, respectively, and neither interferes with the analyte of interest. Compared to the existing OSHA method, the new column provides improved peak shape and faster analysis time.| Market: | Environmental |
| Keywords: | Derivatization, Diethylamine, HPLC, Monoethylamine, NBD-chloride, OSHA Method 41, UV detection |
| Matrix: | N/A |
For Research Use Only. Not for use in diagnostic procedures.
