Plasma Free Metanephrines Quantitation with Automated Online Sample Preparation and Liquid Chromatography-Tandem Mass Spectrometry

Instrument Type: LCMSMS

Plasma free metanephrine (MN) and normetanephrine (NMN), collectively known as Pmets, are preferred biomarkers for pheochromocytoma for clinical research. LC-MS/MS has become widely used to measure Pmets because of its high analytical specificity. Although our recently reported method utilizing LC-MS/MS using IP-SPE and PGC column chromatography is fast and analytically sensitive, it can be further improved by automating the offline sample preparation with online sample preparation technology, which is more time- and cost-effective.