CAN123: Sugars in Honey Using HPAE-PAD: What Is the Best Column?

Instrument Type: IC

Honey is defined by the Codex Alimentarius as the natural, sweet substance produced by honey bees from the nectar of plants or from secretions of living parts of plants or excretions of plant sucking insects on the living parts of plants, which the bees collect, transform by combining with specific substances of their own, deposit, dehydrate, store and leave in the honeycomb to ripen and mature. High-performance anion-exchange chromatography with pulsed amperometric detection is commonly used to determine the carbohydrates in honey. This document evaluates CarboPac columns for that purpose.

AN181: Determination of Streptomycin and Impurities Using HPAE-PAD

Instrument Type: IC

Thermo Scientific Dionex ICS-3000 system with PAD is used to run the USP Compendial Method for the assay of streptomycin sulfate. Key performance parameters, including precision in determining streptomycin purity, limits of detection, linearity, and ruggedness, are shown to be consistent with normal method validation requirement. HPAE-PAD technology is demonstrated here for streptomycin A purity analysis and its feasibility for determinations in a fermentation broth. Finally, streptomycin A purity per the requirements of the ICH for new drug substances is evaluated.