AU167: Determination of Tobramycin in Crude and In-Process Production Samples During Manufacturing Using HPAE-IPAD

Instrument Type: IC

This update describes how tobramycin and its typical impurities can be determined in manufacturing process matrices using the same HPAE-IPAD method described in AN 61. The same method is used to assay tobramycin that is intentionally degraded, demonstrating its suitability as a stability indicating method. An ICS-6000 can be used for this application.

AN179: Carbohydrate and Amino Acid Analysis Using 3-D Amperometry.

Instrument Type: IC

Specific amperometric waveform integration ranges can provide a tool to selectively detect specific compounds. 3-D amperometry enables postchromatographic modification of waveform integration ranges, simplifying the procedure by eliminating the need for multiple injections. This technique can enhance the detection of carbohydrates in the presence of some coeluting amino acids. Additionally, it can minimize some baseline disturbances improving peak integration. Peak area response can conveniently be adjusted to match values from older HPAE-IPAD chromatography systems.