AN270: Determination of Hydroxymethylfurfural (HMF) in Honey and Biomass

Instrument Type: IC

This work describes a high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD)-based method for the determination of HMF in samples ranging from food to treated biomass. A Dionex ICS-3000 system with a CarboPac PA1 column, electrolytically generated hydroxide eluent, and electrochemical detection with disposable Au-on-polytetrafluoroethylene working electrodes are used. The Dionex CarboPac PA1 is a high-capacity, rugged column suitable for determining mono- and disaccharides, and has high resolution for HMF in a wide variety of matrices.