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AN83: Size-Exclusion Chromatography of Polysaccharides with Pulsed Amperometric Detection (PAD).
Instrument Type: ICSize-exclusion chromatography is used in carbohydrate research to determine molecular weight distributions of polysaccharides. Size-exclusion columns separate biological macromolecules by hydrodynamic size. Pulsed amperometric detection (PAD), provides sensitive and specific detection of carbohydrates. Here, fractionated pullulan standards were used to determine the separation range of linear carbohydrates.This application note shows how SEC can be coupled to PAD.
Improved size exclusion chromatography of gum arabic by HPLC-CAD
Instrument Type: HPLC-CADThe Thermo Scientific LC system is applied for size exclusion chromatography analysis of gum arabic. The separation is performed on a Thermo Scientific Acclaim SEC-300 HPLC column, with charged aerosol detection.
