Analysis of Illegal Dyes in Food Matrices Using Automated Online Sample Preparation with Liquid Chromatography-Mass Spectrometry

Instrument Type: LCMSMS

Sudan dyes are red dyes used for coloring solvents, oils, waxes, petrol, or as additives in shoe and floor polish. They have been found in a number of food products such as chili / chili-containing products. Sudan dyes are banned as food additives in the USA, the EU, and many other countries, due to their links to cancer and other negative health effects. To overcome limitations in popular offline SPE and LC-UV measurements, we describe an easy, comprehensive LC method using a Transcend TLX-1 system powered by TurboFlow technology to analyze five illegal dye residues in a variety of sauces.

LC-MS Method Using Cloud Point Extraction for the Determination of Permitted and Banned Azo Colors in Liquid, Semi-liquid and Solid Food Matrices

Instrument Type: LCMS

Colors are food additives that are regulated in the EU, USA, and China. Only approved food colors can be used and others are banned such as some azo compounds, because of their potential carcinogenicity. In this Method, we describe a new Cloud Point Extraction (CPE) method for determining the presence of approved water soluble and banned fat soluble azo colors, followed by separation on a Thermo Scientific Hypersil GOLD column and detection with a Thermo Scientific MSQ Plus single quadrupole mass spectrometer.

Fast analysis of capsaicin in chilies using a Thermo Scientific Acclaim RSLC 120 C18 column

Instrument Type: HPLC

The Thermo Scientific UltiMate 3000 RSLC system is applied for the analysis of capsaicin in chilies. The separation is performed using a Thermo Scientific Acclaim RSLC 120 C18 column, with UV detection at 280 nm.