AN150: Determination of Amino Acids in Cell Cultures and Fermentation Broths.

Instrument Type: IC

This application note describes the use of AAA-Direct to analyze common amino acids in the presence of simple sugars, sugar alcohols, alcohols, and glycols in yeast and bacterial fermentation broths. In this application note, the AminoPac PA10 anion-exchange column is used to separate amino acid and carbohydrate ingredients in cell culture and fermentation broth media. High carbohydrate concentrations can hamper the determination of some amino acids due to coelution. Also in this application note, disposable gold (Au) electrodes are evaluated for use in amino acid determination.