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Related substances analysis of Cefaclor using a C18 HPLC column for EP 8.0 and JP XVI monograph method

Instrument Type: HPLC

The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the related substances analysis of cefaclor. The analysis was performed on a Thermo Scientific Syncronis C18 HPLC column using the method described in the EP 8.0 and JP XVI. The results obtained for resolution and theoretical plates met the criteria stated in the EP/JP. The results obtained for resolution between the peaks due to cefaclor and EP Impurity D was 11.1 (EP criteria of minimum 2.0) and theoretical plates for cefaclor was 1,12,359 (JP criteria of minimum 40,000).

Improved analysis of antibiotics using a Thermo Scientific Hypersil GOLD HPLC column

Instrument Type: HPLC

The Thermo Scientific HPLC system is applied for the analysis of antibiotics. The separation is performed on a Thermo Scientific Hypersil GOLD HPLC column, with UV detection at 254 nm.