TN137: Determination of Carbohydrates in Urine by Capillary HPAE-PAD

Instrument Type: IC

Certain carbohydrates such as mannitol, rhamnose, xylose, 3-O-methylglucose, and lactulose can be determined in urine and serum samples to evaluate disease states in animals and humans. These carbohydrates can be determined directly by HPAE-PAD, a well-established technique that eliminates the need for derivitization, in turn saving time and money. In this work, the separation and detection of the same carbohydrates of interest discussed in AN 1006 are demonstrated using a capillary Dionex CarboPac PA20 anion-exchange column on the ICS-4000 Dedicated Capillary HPIC system.

TN135: Determinations of Monosaccharides and Disaccharides in Beverages by Capillary HPAE-PAD

Instrument Type: IC

Mono- and disaccharide sugar determinations are often used in the food and beverage industry to ensure the quality of a formulated product, to maintain or select for desired sweetness, and to characterize and confirm the source of the carbohydrates (cane, beet, or corn). This application demonstrates the determinations of high concentrations of sugars in beverages using a capillary IC system. Sugars were detected by HPAE-PAD with 4-potential waveform and Au on PTFE working electrode and 0.015” thick gasket.