Quantitative Comparison of Hormones in Drinking Water Between MS/MS and Orbitrap Technology

Instrument Type: LCMSMS

We present a a liquid chromatography – high-resolution, accurate mass (LC-HRAM) methodology using Orbitrap technology as a sensitive, accurate, and reliable alternative to the use of triple quadrupole mass spectrometers in the quantification of hormones in drinking water according to EPA guidelines. By using different scanning modes within the Q Exactive MS, quantitation on precursor ions and identification of fragments ions are possible. These scanning modes can possibly be used for compliance monitoring in place of a triple quadrupole MS.

Qualitative and Quantitative Analysis of Contaminants of Emerging Concern in Biosolids Using a Dilute and Shoot UHPLC-Orbitrap MS Method

Instrument Type: LCMSMS

We develop an HRAM-based LC-MS/MS workflow to quantitatively analyze contaminants of emerging concern (CECs) in biosolids samples and to screen for 381 non-targeted CECs. Unlike LC-QQQ-MS/MS, the Fisher Scientific Exactive Plus Orbitrap full-scan high-resolution, accurate-mass (HRAM) mass spectrometry provides not only quantitative data for targeted compounds but also information on the non-targeted compounds, such as environmental transformation by-products, for possible environmental loadings and ecological effects that would not be available in a targeted assay.