LC-MS/MS Method for the Analysis of Choline and Acetylcholine using a Syncronis HILIC 1.7 µm Column

Instrument Type: LCMSMS

Choline is a water soluble essential nutrient that is produced in the body in small quantities. Choline is a precursor of acetylcholine which is an important neurotransmitter involved in muscle control and memory. Choline is a small, highly polar quaternary amine and will not be retained using a C18 column. The zwitterionic nature of Syncronis HILIC makes it ideal for the retention of this class of compound. This application note demonstrates the successful analysis of acetylcholine and choline using a Syncronis HILIC 1.7 µm column.