Simplified, high-throughput separation of glucocorticoids

Instrument Type: UHPLC

Improved separation of closely related glucocorticoids with reduced method complexity High-throughput analysis possible through a reduced complexity, rapid, two-minute isocratic method Associated reduction in cost per sample through reduced mobile phase consumption and waste generation

EP 8.0 monograph - impurity determination of betamethasone using a C18 HPLC column

Instrument Type: HPLC

The Thermo Scientific Dionex UltiMate 3000 HPLC system is applied for the analysis of Betamethasone and its impurities. The separation was performed on a Thermo Scientific Syncronis C18 column using the method described in the EP 8.0 monograph. The results obtained for resolution between methylprednisolone and betamethasone was 1.55. This exceeds the EP criteria of minimum of 1.5 and the resolution can be further increased by optimising the acetonitrile concentration in mobile phase A in accordance with criteria in the monograph.

Rapid determination of Steroids from urine by LC-MS/MS using Thermo Scientific SOLA HRP SPE and a Thermo Scientific Accucore RP-MS HPLC column

Instrument Type: LCMSMS

The Thermo Scientific HPLC system is applied for the analysis of steroids from urine. The sample was prepared using SOLA HRP solid phase extraction (SPE), and the separation performed on a Thermo Scientific Accucore RP-MS HPLC column in less than 6 minutes, with MS/MS detection. For Research Use Only. Not for use in diagnostic procedures.