Analysis of organic impurities of celecoxib using the method described in the USP

Instrument Type: HPLC

In this work the separation of celecoxib and impurities was performed using the Thermo Scientific BetaBasic Phenyl HPLC column according to the method described in the USP. As the temperature of the column needs to be set to 60 °C the Thermo Scientific Ultimate 3000 LC system was used with a pre-column heater to ensure the mobile phase was at the correct temperature. The resolution obtained between celecoxib and impurity A, and between celecoxib and impurity B met the USP requirements of greater than 1.8.