USP 38 monograph - impurity determination of ampicillin using a C18 selectivity HPLC column

Instrument Type: HPLC

The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the analysis of ampicillin and its impurities. The separation was performed on a Thermo Scientific Hypersil Gold HPLC column using the method described in the USP 38 monograph. All USP criteria was exceeded. Resolution between amoxicillin and ampicillin was 22 (USP criteria of not less than 10). Signal to noise ratio was 140 (USP criteria not less than 3). Tailing factor of ampicillin was 0.8 (USP criteria not more than 1.4) and relative standard deviation for standard solution was 0.04% (USP criteria not more than 10.0%).

EP 8.0 monograph - impurity determination of ampicillin using a C18 HPLC column

Instrument Type: HPLC

The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the analysis of ampicillin and its impurities. The separation was performed on a Thermo Scientific Acclaim 120 C18 HPLC column using the method described in the EP 8.0 monograph. The results obtained for resolution and relative retention time (RRT) exceeded the criteria stated in the EP. Resolution between ampicillin and cefradine was 3.23 (EP criteria minimum of 3.0). RRT with reference to ampicillin and ampicillin dimer in reference solution (d) was 2.84 (EP criteria about 2.8).

Fast analysis of Penicillins using a Thermo Scientific Hypersil GOLD CN HPLC column

Instrument Type: HPLC

The Thermo Scientific HPLC system is applied for the analysis of penicillins. The separation is performed on a Thermo Scientific Hypersil GOLD CN HPLC column, with UV detection at 220 nm.