AN361: Extraction of Total Fat from Food Samples After Acid Hydrolysis Using Accelerated Solvent Extraction with GC-MS Analysis

Instrument Type: Automated Sample Prep

Complex matrices such as food typically require acid hydrolysis or pretreatment prior to solvent extraction. Our newly updated flow-through solvent extraction system allows extraction of matrices which require acidic or alkaline pretreatment. The ability to extract these pretreated matrices significantly expands the capabilities of accelerated solvent extraction technology.This Application Note describes methods used for extraction of total fat from food samples and determination of fat content by FAME (Fatty Acid Methyl Ester) analysis based on AOAC Official Method 996.06 section G.

AN364: Rapid Determination of Total Fat from Dairy Products

Instrument Type: Automated Sample Prep

The U.S. Nutritional Labeling and Education Act of 1990 requires nutritional labels listing the content of saturated and unsaturated fats in food. Accurate determination of fat in certain foods is difficult due to the binding or entrapment of the fat by the matrix. Accelerated Solvent Extraction (ASE) is an automated extraction technique that significantly reduces extraction time and solvent consumption and yields equivalent results. In this work, fat was extracted from sour cream, cream cheese, coffee creamer, heavy whipping cream, and low-fat milk.