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AU72197 : Determination of Tobramycin Using HPAE-IPAD on a Compact IC System: 20 µM tobramycin

Instrument Type: IC

Tobramycin is an important aminoglycoside antibiotic used in ophthalmic and intravenous treatments to treat bacterial infections by blocking protein synthesis.[1] Determinations of tobramycin, kanamycin and other aminoglycoside antibiotics have been previously demonstrated by HPAE-IPAD since 2004.[2–6] This document updates Thermo Scientific™ Application Note 61 using a highpressure-capable Dionex Integrion HPIC system equipped with updated EG technology and an electrochemical detector.

AN159: Determination of Sucralose Using HPAE-PAD

Instrument Type: HPLC (Biocompatible)

This application note describes the use of HPAE- PAD to determine sucralose in food and beverage samples. Red Raspberry Diet Rite is a soft drink artificially sweetened with sucralose. It also contains natural and artificial flavors, organic compounds (potassium benzoate and citrate), food dye, and another nonnutritive sweetener (acesulfame potassium). These model food and beverage samples present a great challenge for most chromatographic methods. In this note, the CarboPac™ PA20 anion-exchange column is used to separate sucralose from other ingredients in Red Raspberry Diet Rite and Splenda.