Quantitation of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 in Plasma for Clinical Research Using the Prelude SPLC and TSQ Endura Mass Spectrometer

Instrument Type: LCMSMS

LC-MS methods have been widely used to analyze vitamin D2 and vitamin D3 25-hydroxy metabolites (25OH D2 and 25OH D3) in human plasma. Clinical research laboratories are always seeking fast and cost-effective methods to improve analytical efficiency. Here we evaluated a high-efficiency, simple sample preparation method implemented on a Prelude SPLC system that combines online sample cleanup powered by Thermo Scientific TurboFlow technology with chromatographic separation and a TSQ Endura triple quadrupole mass spectrometer.