A robust, sensitive, and reliable multi-residue LC-MS/MS method workflow solution that uses the Thermo Scientific TSQ Altis triple quadrupole mass spectrometer provides analysis and quantitation of more than 160 veterinary drugs with the Thermo Scientific VetDrugs Explorer Collection solution in salmon (fillet), bovine muscle, and milk. The complete instrument and data processing method including SRM settings is included with the VetDrugs Explorer Collection start-to-finish workflow solution. In 17 minutes, the target veterinary drugs were detected and quantified in food matrices.

The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the organic impurities analysis of Sulfacetamide Sodium. The analysis was performed on a Thermo Scientific Syncronis aQ HPLC column using the method described in the USP 39 monograph. The results obtained for resolution, tailing factor and %RSD met the criteria stated in the USP. The results obtained for resolution between sulfacetamide and sulfanilamide was 21 (USP criteria of NLT 5.0), tailing factor for sulfacetamide was 0.98 (USP criteria of NMT 1.5) and %RSD for sulfacetamide was 1.0% (USP criteria of NMT 2.0%).

The Thermo Scientific Dionex UltiMate 3000 LC system is applied for assay analysis of sulfacetamide Sodium. The analysis was performed on a Thermo Scientific Syncronis aQ HPLC column using the method described in the USP 39 monograph. The results obtained for tailing factor and %RSD met the criteria stated in the USP. The results obtained for the tailing factor for sulfacetamide was 0.94 (USP criteria was 0.9 to 1.8) and %RSD for sulfacetamide was 0.1% (USP criteria was NMT 0.5%).

The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the related substances analysis of Sulfacetamide Sodium. The analysis was performed on a Thermo Scientific Syncronis aQ HPLC column using the method described in the EP 8.0. The results obtained for resolution met the criteria stated in the EP. The results obtained for resolution between the peaks due to Impurity A and sulfacetamide was 14.5 (EP criteria of minimum 5.0).

This application shows the advantages of using the Thermo Scientific™ Hypersil GOLD aQ VANQUISH™ 1.9 µm column and Thermo Scientific™ Vanquish™ UHPLC system, for the fast analysis of sulfur-containing drugs. The built-in method scaling of the Chromeleon software and high pressure capabilities of the Hypersil GOLD VANQUISH columns, that allow operation at higher flow rates,enable the development of rapid analytical methods whilst maintaining high performance.