Development of a sensitive and reproducible method for analysis of 22 underivatized amino acids and application of the method to quantify 17 amino acids in wine. Accurate quantification of proline, using an isotopically labeled internal standard.
Specific amperometric waveform integration ranges can provide a tool to selectively detect specific compounds. 3-D amperometry enables postchromatographic modification of waveform integration ranges, simplifying the procedure by eliminating the need for multiple injections. This technique can enhance the detection of carbohydrates in the presence of some coeluting amino acids. Additionally, it can minimize some baseline disturbances improving peak integration. Peak area response can conveniently be adjusted to match values from older HPAE-IPAD chromatography systems.
The Thermo Scientific HPLC system is applied for the analysis of PTH amino acids. The separation is performed on a Thermo Scientific Hypersil GOLD HPLC column, with UV detection at 269 nm.
The Thermo Scientific UtliMate 3000 HPLC system is applied for the analysis separation of theanine and amino acids in green tea. The separation is performed on a Thermo Scientific Acclaim PolarAdvantage HPLC column, with UV detection at 330 nm.
