To demonstrate the application of a software-based method development approach for the advanced separation of all known water-soluble vitamins in reversed-phase mode with ultraviolet detection. To achieve transferability of the predicted method with excellent agreement of predicted and experimental retention times.
To describe a workflow for the simultaneous quantitative analysis of fatsoluble and water-soluble vitamins in drinks and food supplement tablets using the Thermo Scientific™ Vanquish™ Flex Duo UHPLC system and the Thermo Scientific™ Acclaim™ Polar Advantage II column.