In this paper, a simple and efficient HPLC method was established by using Vanquish Core high performance liquid chromatography (Vanquish Core) combined with general quality detector-Charged Aerosol Detection(CAD). It can meet the needs of simultaneous determination of Poloxam 188 and Tween 80 in adeno-associated virus (AAV) vector preparation. The method has good stability and high sensitivity. The wide linear range is suitable for the determination of different concentrations of samples, and can be used as an effective means for the control of excipient residues in drugs such as Poloxam 188
