The Thermo Scientific Dionex UltiMate 3000 RSLC system is applied for the analysis of ursolic acid in apple peel. The separation is performed on a Thermo Scientific Acclaim C30 column, with UV detection at 210 nm. Chromatogram A is extracted Fuji apple, chromatogram B is extracted red delicious apple and chromatogram C is standards at 100 μg/mL.
The Thermo Scientific UltiMate 3000 HPLC system is applied for the analysis of ursolic and oleanolic acids. The separation is performed on a Thermo Scientific Acclaim 120 C18 and an Acclaim C30 HPLC column, with UV detection. The results are compared.