The straightforward transfer from a Shimadzu Nexera-i system to a Thermo Scientific Vanquish Core HPLC system is demonstrated for the EP method for chlorhexidine impurity analysis. Equivalent chromatographic outcomes are provided by the two systems with improved system precision of the Vanquish Core HPLC system. Small deviations of absolute retention times due to different system gradient delay volumes were easily decreased by an adjustment of the idle volume of the Vanquish Core autosampler.
Straightforward transfer of an EP monograph HPLC method from a Thermo Scientific™ UltiMate™ 3000 Standard HPLC system to a Thermo Scientific™ Vanquish™ Core HPLC system is demonstrated. Equivalent chromatographic results are obtained with both systems, but an improved resolution and retention time stability are provided by the Vanquish Core HPLC system.
Seamless transfer of an EP monograph HPLC method from a Waters™ Alliance™ HPLC system to a Thermo Scientific™ Vanquish™ Core HPLC system is demonstrated. Equivalent chromatographic results are obtained with both systems, but improved resolution and system repeatability are provided by the Vanquish Core HPLC system.
The application demonstrates the simple transfer of a compendial LC method for the analysis of chlorhexidine impurities from an Agilent 1260 Infinity LC system to a Vanquish Core HPLC system. Equivalent chromatographic results are obtained with both instruments. Enhanced hardware features of the Vanquish Core HPLC system enable the flexible adjustments of the overall system gradient delay volume, which facilitates fine-tuning of retention time matches during the transfer.
To demonstrate how custom injection programs affect chromatographic parameters such as asymmetry, resolution, and efficiency of a selected EP method.
