A platform using Dionex ion chromatography (IC) coupled to triple quadrupole mass spectrometry (MS) was developed to measure various metabolites in Brassica napus seeds. The IC tandem mass spectrometry (IC-MS/MS) technique provides a good tool to identify and quantify metabolites in a complex matrix.
The Thermo Scientific UltiMate 3000RS system is applied for the analysis of sugar phosphates. The separation is performed using a Thermo Scientific GlycanPac AXH-1 1.9µm column, with charged aerosol detection. The Thermo Scientific GlycanPac AXH-1 is a column designed to classify anionic carbohydrates by charge and resolve them by HILIC interactions. For this group of simple sugar phosphates, an isocratic analysis is sufficient using a volatile mobile phase that is compatible with charged aerosol detection or mass spectrometry.
