The Thermo Scientific Dionex Ultimate 3000 BioRS system in combination with the Thermo Scientific Accucore 150-Amide-HILIC is used to run separation of 2-AB-labelled dextran ladder in gradient mode. The column compartment was set at different temperature between 20 and 50 °C. At high temperature the selectivity between oligomers was improved, and peaks were in average narrower. Consequently higher peak capacity was achieved at high temperature.
The Thermo Scientific Dionex UltiMate 3000 BioRS is applied for separation of 2-aminobenzamide labeled dextran ladder. The HILIC separation is achieved with Thermo Scientific Accucore 150-Amide-HILIC 2.6 µm, 150 x 2.1 mm.The Glucose Units (GU) values are easily assigned the dextran ladder peaks. A calibration curve logarithm GU versus retention time is automatically created with Thermo Scientific Dionex Chromeleon 7.2 Chromatography Data System. The calibration curve is used to assign GU value to unknown glycans.
