Red wine is a rich source of phenolic antioxidants and is reported to reduce the risk of diabetes, cancer, Alzheimer's disease, and cardiovascular disease. To improve grape yields it is common practice in vineyards to use pesticides, such as fungicides and insecticides. This application presents a fast, easy, and cost-effective method for the determination of 24 pesticide residues in red wine using the QuEChERS extraction method (AOAC version), cleanup by dispersive solid-phase extraction (dSPE), followed by LC-MSMS analysis.
European Regulation 396/2005 sets maximum residue levels of pesticides in different products of plant and animal origin. These regulations present a significant analytical challenge with respect to LOQs required for some specified food matrices such as baby food. The multi-residue pesticide method described can be applied to fruits, cereals and composite baby foods at LODs in the range of 0.8–10.3 µg/kg - below respective EU maximum residue limits (MRLs). The method is validated for 48 pesticides from different classes, but can be readily extended to a larger number of residues.
Forty-four multi-class veterinary drugs of known concentrations were employed to demonstrate how the use of a generic variable data-independent acquisition (vDIA) method with wide MS/MS precursor isolation windows achieves sensitivity and selectivity comparable to data-dependent MS2 acquisition (using narrow isolation windows) in quantitative and qualitative small molecule applications. In addition, a full record of MS and MS/MS data for the measured sample fit for non-targeted and unknown screening purposes is delivered. vDIA is not available in the United States of America
The Thermo Scientific UltiMate 3000 HPLC system is applied for the determination of 24 pesticide residues in red wine. The samples are prepared using Thermo Scientific HyperSep Dispersive SPE (QuEChERS), and the separation performed using a Thermo Scientific Accucore aQ HPLC column, with MS/MS detection.
