GOS occur naturally in the milk of many animals including humans, cows, and wallabies. GOS are primarily composed of galactose and often terminate with a glucose residue at the reducing end. These carbohydrates are enzymatically produced by transgalactosylation reactions of lactose catalyzed by β-galactosidases and give rise to galactose oligomers. Separation of individual oligosaccharides in GOS was achieved on a Thermo Scientific™ Dionex™ CarboPac™ PA200 column. Oligosaccharide detection was by PAD with a gold working electrode, and therefore no sample derivation was required.
To demonstrate a simple, cost-effective analytical solution for the routine determination of low level acrylamide in food and coffee samples, from sample extraction to detection and quantification, using a Thermo Scientific™ ISQ™ 7000 GC-MS system coupled with a Thermo Scientific™ TRACE™ 1310 Gas Chromatograph and Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS) software.
To demonstrate the applicability of the Thermo Scientific™ iCAP™ RQ ICP-MS with Argon Gas Dilution (AGD) for performing direct, high-throughput analysis of relevant trace elements in complex environmental samples (sea water, waste water, or drinking water).
Potassium bromate is a food additive used as “flour improver” in the baking industry. Bromate is considered a carcinogenic and nephrotoxic substance. We developed a method for a selective and sensitive determination of bromate in flour that uses IC coupled with MS. A recently introduced high-capacity anion-exchange column was used to separate bromate from matrix anions. Six commercial flour and flour products including homemade bread baked using flour containing potassium bromate, were analyzed. LOD and LOQ of bromate in the prepared solution were 0.10 µg/L and 0.34 µg/L, respectively.
The Thermo Scientific Dionex Ultimate 3000 system is applied for the peptide mapping analysis of an Fusion Protein (Aflibercept). The separation was performed on a Thermo Scientific Accucore vanquish C18+ column by using a Thermo Scientific SMART digest kit at UV detection of 210 nm. The SMART Digest kit provides significant improvements in reproducibility, which results in fewer sample failures, higher throughput and the ability to more easily interrogate data.
