α- and β-bitter acids in hops were determined using the Thermo Scientific™ Vanquish™ Online SPE UHPLC system. Online SPE with a Hypersil GOLD™ C8 column (20 × 2.1 mm) eliminates the need for standalone SPE cartridges, while separation of α- and β-acids and their derivatives is achieved using a Hypersil GOLD™ C18 Selectivity column.
Evaporation tube temperature, CDM temperature, and coupling mode optimized.
Cytochrome C digest analysis highlighting HPG sync
This application brief demonstrates the performance of the Thermo Scientific Dionex CarboPac PA300-4um column for high-efficiency, high-resolution carbohydrate analysis using HPAE-PAD. The column effectively separates neutral and charged carbohydrates, including sugars, inulin, and alditols. Results show this product as an ideal first-pass column for carbohydrate method development, complementary to the CarboPac PA200.
We developed a sensitive and automated method using CIC to determine both TOF and EOF in FCM. The TOF method is beneficial for manufacturers to comply with current state regulations. We also employed a non-targeted LC-HRMS approach, which allowed us to detect 46 PFAS compounds in the EOF fraction, many of which fall outside of the typical list of targeted screening or quantification methods.
