A time and labor-saving online sample preparation workflow by amino acid derivatization in the autosampler's needle is presented for medium and highly complex sample matrices (beverages, food, and fertilizers) analyzed at one external and two in-house laboratories. Robust and reproducible method performance, column stability over several hundred injections, and applicability at different instrument tiers are highlighted.
This technical note explores the advantages of using relative standard error (RSE), weighted least squares (WLS) approximations, and inverted calibrations to enhance calibration quality in chromatography. By optimizing calibration curves through quadratic fitting, offset adjustments, and suitable weighting factors (such as 1/Amount²), the calibration process can be significantly improved, resulting in lower RSEs and better overall calibration quality. Incorporating RSE as an evaluation tool offers a deeper understanding of calibration reliability.
HPAE-PAD is a well-established technique for separating and quantifying a wide range of carbohydrates, providing high resolution separation and high sensitivity. This document discusses the basics of the separation and detection mechanisms and limitations of HPAE-PAD, good practices and techniques for successful analyses.
The proper preparation and use of eluents are the most crucial parts for success in carbohydrate analysis with ion chromatography with PAD detection. This document describes purity criteria for reagents, proper eluent storage, proper use, identification of contaminated eluent and consequences.
Industries are adopting methods to enhance analytical performance while minimizing ecological impact. In ion chromatography, using microbore columns with diameters of 2 mm or less reduces eluent use, cutting hazardous waste and improving throughput.This note highlights rapid anion analysis in water using microbore column, which significantly reduces liquid waste compared to standard bore columns. This method enhances safety and sustainability by minimizing waste production.Both chemical and electrolytic suppression techniques are demonstrated.
