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Chromatographic method for aggregate analysis of Aflibercept i.e fusion protein using SEC column.

Instrument Type: HPLC (Biocompatible)

Analysis of protein aggregation of Aflibercept i.e fusion protein by size-exclusion chromatography, showing the universal applicability of the Thermo Scientific MAbPac SEC-1 column for aggregate analysis of fusion protein. Aggregate analysis is major CQA for protein based drugs and requires tight monitoring and control.

Measuring alcohol biomarkers from urine - a splitting headache!

Instrument Type: LCMSMS

Overcoming a significant matrix effect causing peak splitting during the analysis of the minor ethanol metabolites; ethyl sulphate and ethyl glucoronide.

Superior separation of nicotine and tobacco related alkaloids by utilizing the selectivity of HILIC

Instrument Type: LCMSMS

HPLC-MS/MS separation for the tobacco alkaloids nicotine, nornicotine, cotinine, norcotinine, trans-3’-hydroxycotinine, and anabasine.

AN73267: Determination of Perchlorate in Drinking Water using Ion Chromatography

Instrument Type: IC

This application note updates the method with a high resolution IonPac AS16 4µm-2mm column. In comparison to the IonPac AS16 column, the IonPac AS16-4µm column exhibits higher peak efficiency while maintaining the same selectivity. This application note shows that using AS16-4µm column saves 3 min per injections, thereby improving productivity in perchlorate determinations in drinking water. Key performance parameters were evaluated including separation, linearity, limits of detection, accuracy, and precision. Perchlorate concentrations were determined in three drinking water samples.

Flexible HPLC instrument setups for double usage as one heart-cut-2D-LC system or two independent 1D-LC systems

Instrument Type: LCMS

The Technical Note describes how to setup single heart-cut 2D-LC instruments with the Vanquish platform, applying solvent strength reduction and trapping of the cut fraction. In addition instruments are described, which can be flexibly utilized either as one heart-cut 2D-LC instrument or as two 1D-LC instruments.