This white paper investigates the impact of vial surface chemistry on analyte recovery in LC and LC-MS workflows, with a focus on adsorption-sensitive biomolecules at low concentrations. It compares different vial materials using representative analytes—including peptides, proteins and monoclonal antibodies—to evaluate how nonspecific binding affects recovery and reproducibility. The study demonstrates that selecting an appropriate vial surface can significantly reduce analyte loss and improve confidence in results, providing practical guidance for optimizing sample handling in routine work.
Natural gas, an important energy source, is often compressed into liquid for easier transportation. Combustion-IC (C-IC) has been previously demonstrated as an ideal approach to eliminate the sample matrix and increase sample homogeneity for various sample matrices. Additionally, C-IC is recognized as an ASTM standardized method (D7994) to determine halogens and sulfur in LPG. Here, we determine halides and sulfur species in LPG using C-IC, leveraging advances in the Thermo Scientific™ Cindion™ C-IC system using the Thermo Scientific™ Cindion™ LPG/Gas Module with built-in safety features.
Analysis of large RNA using an ion-pair reversed-phase method on a SurePac Oligo RP MDi column<enter next 255 characters of description><enter final 90 characters of description>
This application note demonstrates a USP-aligned ion chromatography method for quantitative assay of magnesium sulfate using the Dionex IonPac CS16 column and NGES-C electrolytic suppressor. The method uses suppressed conductivity detection with 48 mM MSA and meets USP system suitability criteria, including Mg/Ca resolution, tailing factor, and %RSD. Linearity was confirmed from 10–300 µg/mL, and sample assays fell within 98–102% with low RSDs. The NGES-C improves baseline stability, noise reduction, and robustness for routine pharmaceutical QC.
This application note presents an ion chromatography method for trace nitrite and nitrate analysis in lactose monohydrate and colloidal silicon dioxide. Using a Dionex IonPac AS19-4µm column and NGES-A suppressor, the method achieved sensitive, selective separation with LOQs of 0.1 µg/g nitrite and 0.2 µg/g nitrate. Strong linearity, precision, and recovery support its use in USP-aligned nitrosamine risk assessment for pharmaceutical excipients
