Iodine is an essential mineral for our body. This application note demonstrates the development and validation of a 15 min IC-ED method for the determination of iodide in multivitamin products. The method uses a 2 mm Dionex IonPac AG20/AS20 column set with electrolytically generated potassium hydroxide (KOH) eluent and a disposable silver working electrode. Using this method, iodide is resolved from all common anions and organic acids found in the multivitamin samples. The method has a linear calibration, is sensitive, precise, and accurate.
We demonstrate a workflow combining chemical release of O-linked glycans, followed by sample cleanup and analysis by HPAE-PAD hyphenated to a Q Exactive HF Hybrid Quadrupole-Orbitrap MS. A recently introduced Dionex CarboPac PA300-4μm column, enables simultaneous separation of neutral and charged glycans without the need for derivatization. High-resolution MS data and MS/MS spectra with diagnostic fragment ions provide reliable structural annotations of heterogeneous glycans. We applied HPAE-PAD/MS to analyze O-glycan structures released from four different glycoproteins.
Demonstrate the performance of the Thermo Scientific™ Vanquish™ Neo UHPLC system, the next−generation nano-, capillary- and micro-flow LC, coupled to a Thermo Scientific™ Orbitrap Exploris™ 480 mass spectrometer for high-throughput bottom−up proteome profiling using the 75 μm I.D. × 15 cm Thermo Scientific™ EASY-Spray™ PepMap™ Neo column
This record has QAR conditions and results for the 2 mm Dionex IonPac CS19-4µm column. The column must be pre-conditioned offline with acetonitrile and methanesulfonic acid (MSA). See instructions in the column manual (attached). The column is optimized for small, hydrophilic amines such as ethanolamines, methylamines, ethylamines, as well as the biogenic amines.
Demonstrate the superior performance of the Thermo Scientific™ Vanquish™ Neo, next-generation low-flow UHPLC system, for nanoLCMS bottom-up proteome profiling when coupled to a Thermo Scientific™ Orbitrap Exploris™ 480 mass spectrometer using the 75 μm I.D. × 75 cm Thermo Scientific™ EASY-Spray™ PepMap Nep column. Demonstrate system versatility and potential for new levels of proteomic depth coverage through the coupling of two 75 cm long nano columns.
