Cytochrome C digest analysis highlighting HPG sync
This application brief demonstrates the performance of the Thermo Scientific Dionex CarboPac PA300-4um column for high-efficiency, high-resolution carbohydrate analysis using HPAE-PAD. The column effectively separates neutral and charged carbohydrates, including sugars, inulin, and alditols. Results show this product as an ideal first-pass column for carbohydrate method development, complementary to the CarboPac PA200.
We developed a sensitive and automated method using CIC to determine both TOF and EOF in FCM. The TOF method is beneficial for manufacturers to comply with current state regulations. We also employed a non-targeted LC-HRMS approach, which allowed us to detect 46 PFAS compounds in the EOF fraction, many of which fall outside of the typical list of targeted screening or quantification methods.
The switchable heart-cut 2D-LC system facilitates the straightforward at-line analysis of mAb producing cell culture samples for the upstream monitoring of titer, charge variants, and size variants of the intact antibody. While the first dimension purifies the mAb from matrix components by Protein A affinity chromatography, selectable second dimension mechanisms enable the assessment of the aggregation level by SEC or the abundance monitoring of charge variants by SCX.
Demonstrating the performance of the Thermo Scientific Dionex NGES Anion Suppressor (NGES-A) for the implementation of US EPA Method 332.0, highlighting the increased robustness and resilience compared to the previous suppressor model.Design enhancements of the new Dionex NGES suppressor ensure reliable analyses to meet the growing demands of the IC hyphenated solutions such as IC-MS.EPA 332.0
