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Improved separation of PEG-20K labeled with CY5 using the Thermo Scientific Acclaim SEC-300 HPLC column
Description
In this work, polyethylene glycol mono-methyl ether has been labeled with CY5. The Thermo Scientific Acclaim SEC-300 column separates the labelled PEG from the reaction byproducts using size exclusion, but does not resolve labeled from unlabeled PEG. The CY5-labeled PEG is detected by both the FLD and UV; in contrast the CAD detects the labeled and unlabeled PEG equally. Both the UV and CAD detect some of the synthetic by-products. Using multiple detectors allows the reaction yield to be calculated.| Market: | Chemical |
| Keywords: | Acclaim SEC-300, Charged aerosol detection, Cyanine dye, Fluorescence detection, PEG, UV detection |
| Matrix: | N/A |
For Research Use Only. Not for use in diagnostic procedures.
