Pharmacopeial assay and related substances analysis of minocycline hydrochloride using a C8 HPLC column
DescriptionThe Thermo Scientific Dionex UltiMate 3000 LC system is applied for the assay and related substances analysis of minocycline hydrochloride. The analysis was performed on a Thermo Scientific Hypersil GOLD C8 HPLC column using the method described in the EP 8.0. The results obtained for resolution and theoretical plates met the criteria stated in the EP. The results obtained for resolution between the peaks due to impurity A and minocycline was 4.3 (EP criteria of minimum 2.0) and theoretical plates for minocycline (0.125 mg/mL) was 6376 (EP criteria of minimum 3000).
|Keywords:||HPLC, Hypersil GOLD C8, UV detection, EP 8.0 monograph, minocycline, tetracycline antibiotic|
|Affiliation:||Thermo Fisher Scientific, APAC CoE Lab, Ahmedabad, India|
For Research Use Only. Not for use in diagnostic procedures.