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High-Throughput LC-MS/MS Quantification of 17-Hydroxyprogesterone (17-OHP) in Human Blood Serum for Clinical Research Purposes
Description
17-Hydroxyprogesterone (17-OHP) is a biosynthetic precursor to other steroids such as corticosteroids, androgens, and estrogens. It is converted to 11-deoxycortisol by 21-alpha-hydroxylase or to androstenedione by 17, 20 lyase. Researchers investigating how these enzymes function need to measure 17-OHP within an analytical range of 10 to 1,000 ng/dL (0.3 to 30 nmol/L) in blood serum. 17-OHP readily forms positive ions by atmospheric-pressure chemical ionization (APCI), which is less prone to matrix effects than electrospray ionization (ESI).| Market: | Clinical Research |
| Keywords: | Steroid, TSQ Endura, High-throughput, Transcend, multichannel, triple quadrupole MS, 17-hydroxyprogesterone, 17-OHP |
| Matrix: | Blood serum |
| Author: | Joe DiBussolo, Marta Kozak |
| Affiliation: | Thermo Fisher Scientific |
For Research Use Only. Not for use in diagnostic procedures.
