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High-Throughput LC-MS/MS Quantification of Estrone (E1) and Estradiol (E2) in Human Blood Plasma/Serum for Clinical Research Purposes
Description
Estrone (E1) and estradiol (17-beta-estradiol or E2) are two of several estrogens involved in the development and function of female anatomical and physiological characteristics and processes such as the menstrual cycle. Researchers seek to quantify E1 and E2 within an analytical range of 5 to 500 pg/mL (18.5 to 1,850 pmol/L) in blood plasma or serum. E1 and E2 form negative ions by deprotonation in both ESI and APCI MS sources. In order to robustly achieve the needed quantitation limits, most researchers use dansyl chloride to form positively charged derivatives of these and other estrogens.| Market: | Clinical Research |
| Keywords: | Estrogens, Estrone, Steroid, Estradiol, TSQ Endura, Transcend, E1, E2 |
| Matrix: | Blood serum |
| Author: | Joe DiBussolo, Marta Kozak |
| Affiliation: | Thermo Fisher Scientific |
For Research Use Only. Not for use in diagnostic procedures.
