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High-Throughput LC-MS/MS Quantification of Pregnenolone in Human Blood Serum for Clinical Research Purposes
Description
Pregnenolone is a biosynthetic precursor to other steroids such as corticosteroids, androgens, and estrogens. Researchers investigating conversion to progesterone or to 17-OH-pregnenolone need to quantify pregnenolone within an analytical range of 0.3 to 1.5 nmol/L in blood serum. Since pregnenolone does not ionize well, derivatization with hydroxyl amine was necessary to reliably achieve the desired detection. We pursued this strategy along with liquid-liquid extraction to develop a simple sample preparation that would provide robust and sensitive quantitation of pregnenolone using ESI.| Market: | Clinical Research |
| Keywords: | Steroid, Quantitation, TSQ Endura, High-throughput, pregnenolone, Transcend, multichannel, triple quadrupole MS |
| Matrix: | Blood serum |
| Author: | Joe DiBussolo, Marta Kozak |
| Affiliation: | Thermo Fisher Scientific |
For Research Use Only. Not for use in diagnostic procedures.
