HPAE-PAD is a well-established technique for separating and quantifying a wide range of carbohydrates, providing high resolution separation and high sensitivity. This document discusses the basics of the separation and detection mechanisms and limitations of HPAE-PAD, good practices and techniques for successful analyses.
The proper preparation and use of eluents are the most crucial parts for success in carbohydrate analysis with ion chromatography with PAD detection. This document describes purity criteria for reagents, proper eluent storage, proper use, identification of contaminated eluent and consequences.
Industries are adopting methods to enhance analytical performance while minimizing ecological impact. In ion chromatography, using microbore columns with diameters of 2 mm or less reduces eluent use, cutting hazardous waste and improving throughput.This note highlights rapid anion analysis in water using microbore column, which significantly reduces liquid waste compared to standard bore columns. This method enhances safety and sustainability by minimizing waste production.Both chemical and electrolytic suppression techniques are demonstrated.
The Thermo Scientific UltiMate 3000 RS HPLC System is applied for thechiral purity of Propaquizafop. The analysis was performed on a Thermo Scientific Hypersil Chiral OT HPLC column using
To demonstrate the suitability of the Thermo Scientific™ TSQ™ 9610 triple quadrupole GC-MS/MS system with Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS) software for the routine and regulatory compliance testing of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzo-p-furans (PCDD/Fs) in food and feed. Analysis of food and feed samples previously analyzed by the European Reference Laboratory for POPs (Freiburg, Germany) and the Wageningen Food Safety Research laboratory (Wageningen, Netherlands) was carried out to ensure results were consistent.