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Determination of Isohumulones using Rapid Separation On-Line SPE
Description
The BeerNHop solution for high throughput was applied to analyze the Thermo Scientific isohumulones standard. Automated analyte enrichment was done by on-line solid phase extraction (SPE) on a Thermo Scientific Hypersil GOLD C8 column. Then, the separation was performed on a Hypersil GOLD 1.9 µm column with UV detection at 270 nm. With a total run time of 9 minutes, the method allows very fast screening for isohumulones in beer. Hence, beer bitterness is quantified and the isohumulone pattern is identified in a matter of minutes.| Market: | Food and Beverage |
| Keywords: | Beer, BeerNHop, Bitterness, Brewery, Brewing, Hop, Isohumulones |
| Matrix: | Certified isohumulones standard in methanol (100 µg/mL) |
For Research Use Only. Not for use in diagnostic procedures.
