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Determination of Humulones and Lupulones using Standard On-Line SPE
Description
The BeerNHop solution using an on-line SPE-UHPLC method was applied for the analysis of the Thermo Scientific tetrahydroisohumulones standard. Automated analyte enrichment was done by solid phase extraction (SPE) on a Thermo Scientific Hypersil GOLD C8 column. Then, the separation was performed on a Hypersil GOLD 3 μm column with UV detection at 270 nm. With a total run time of 20 minutes, the method allows fast screening for remaining humulones (or lupulones). Hence, the isomerization progress during beer brewing can be observed fast and easy.| Market: | Food and Beverage |
| Keywords: | Beer, BeerNHop, Brewery, Brewing, Hop, Humulones, Isohumulones, Wort |
| Matrix: | Certified humulones, Lupulones standard in methanol (100 µg/mL) |
For Research Use Only. Not for use in diagnostic procedures.
