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Determination of Tetrahydroisohumulones using Standard On-Line SPE
Description
The BeerNHop solution using an on-line SPE-UHPLC method was applied for the analysis of the Thermo Scientific tetrahydroisohumulones standard. Automated analyte enrichment was done by solid phase extraction (SPE) on a Thermo Scientific Hypersil GOLD C8 column. Then, the separation was performed on a Thermo Scientific Hypersil GOLD 3 μm column with UV detection at 270 nm. With a total run time of 20 minutes, the method allows fast screening for reduced isohumulones in beer. Hence, beers can be screened if they comply with the German purity law.| Market: | Food and Beverage |
| Keywords: | a-acids, Beer, BeerNHop, Bitterness, Brewery, Hop, Isohumulones |
| Matrix: | Certified tetrahydroisohumulones standard in methanol (100 µg/mL) |
For Research Use Only. Not for use in diagnostic procedures.
