AN361: Extraction of Total Fat from Food Samples After Acid Hydrolysis Using Accelerated Solvent Extraction with GC-MS Analysis

Instrument Type: Automated Sample Prep

Complex matrices such as food typically require acid hydrolysis or pretreatment prior to solvent extraction. Our newly updated flow-through solvent extraction system allows extraction of matrices which require acidic or alkaline pretreatment. The ability to extract these pretreated matrices significantly expands the capabilities of accelerated solvent extraction technology.This Application Note describes methods used for extraction of total fat from food samples and determination of fat content by FAME (Fatty Acid Methyl Ester) analysis based on AOAC Official Method 996.06 section G.

AN112: Determination of Nitrate and Nitrite in Meat Using High-Performance Anion-Exchange Chromatography

Instrument Type: IC

Nitrate and nitrite are added to processed meat products to protect against microorganisms. However, nitrite can react with secondary amines to form nitrosoamines, a class of carcinogenic compounds. This Application Note describes an accurate and sensitive method in which nitrate and nitrite are extracted from meat products and then determined directly using anion exchange chromatography with UV detection. Commercially available ham and salami were used as model samples.