Label free analysis of amino acids using UHPLC with charged aerosol detection

Instrument Type: UHPLC

Amino acids present in protein formulations serve as buffers, bulking agents, stabilizers, and antioxidants. Eight specific amino acids are commonly used as excipients in protein therapeutic formulations due to their physicochemical properties. These include Arginine, Aspartic acid, Glutamic acid, Lysine, Proline, Glycine, Histidine, and Methionine. The UHPLC column, an Acclaim PA2 column, provides excellent separation characteristics for these un-labeled amino acids. Gradient conditions are adjusted by selecting appropriate ion-pairing reagents, pH and level of organic solvents.