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AN123: Determination of Inorganic Anions and Organic Acids in Fermentation Broths

Instrument Type: IC

Fermentation broths are complex mixtures of nutrients, waste products, cells and cell debris, and desired products. Fermentation monitoring is important for many industries because carbon sources and metabolic by-products can impact the yield of the desired products. This application note describes the use of two different anion-exchange columns, IonPac AS11 and IonPac AS11-HC, with suppressed conductivity detection, to analyze common organic and inorganic anions in yeast and bacterial fermentation broths.

High resolution separation of Cytochrome C from three different species

Instrument Type: HPLC

The Thermo Scientific HPLC system is applied for the analysis of Cytochrome C from three different species (yeast, equine and bovine). The separation is performed using a Thermo Scientific Acclaim 300 C18 column, with UV detection at 214 nm.

Improved separation of phospholipids derived from biological extracts using a Thermo Scientific Accucore RP-MS column

Instrument Type: LCMS

The Thermo Scientific HPLC system is applied for the analysis of phospholipids derived from biological extracts. The separation is performed on a Thermo Scientific Accucore C18 column, with MS detection.