Related Compound analysis of Lactulose as per USP monograph

Instrument Type: HPLC

The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the analysis of Lactulose. The separation was performed on a Thermo Scientific Hypersil Gold amino HPLC column using the method as described in the USP 38 monograph. Resolution between Epilactose and Lactose Peak was 1.28 (met USP criteria of NLT 0.9), RRT for Epilactose with respect to Lactulose peak was 0.92 (met USP criteria of about 0.83), Resolution between Lactulose and Lactose Peak was 3.17 (met USP criteria of NLT 1.5), RRT for Lactose with respect to Lactulose peak was 1.22 (met USP criteria of about 1.1),