AN115: Determination of Trifluoroacetic Acid (TFA) in Peptides

Instrument Type: IC

Trifluoroacetic acid (TFA) is used in the manufacturing process to release synthesized peptides and during reversed-phase HPLC purification of peptides. This application is to evaluate peptides for residual TFA, fluoride, and acetate in commercially prepared peptides using ion chromatography with the IonPac AS14 column and suppressed conductivity detection. This method requires minimal sample preparation, and the analytes, fluoride, acetate, and TFA, are easily separated without significant peptide interference. See AN160 for an RFIC method.