AN159: Determination of Sucralose Using HPAE-PAD

Instrument Type: HPLC (Biocompatible)

This application note describes the use of HPAE- PAD to determine sucralose in food and beverage samples. Red Raspberry Diet Rite is a soft drink artificially sweetened with sucralose. It also contains natural and artificial flavors, organic compounds (potassium benzoate and citrate), food dye, and another nonnutritive sweetener (acesulfame potassium). These model food and beverage samples present a great challenge for most chromatographic methods. In this note, the CarboPac™ PA20 anion-exchange column is used to separate sucralose from other ingredients in Red Raspberry Diet Rite and Splenda.