Faster analyss of NBD derivatives of volatile alkylamines using a Thermo Scientific Acclaim C30 HPLC column

Instrument Type: HPLC

The Thermo Scientific Acclaim C30 resolved four common volatile amines with simple, isocratic reversed phase conditions. In this case, 4-chloro, 7-nitro, 1,2,3-benzoxadiazole (NBD-chloride) reacts with amines in the trap, to produce a highly-colored, fluorescent derivative. The unreacted reagent elutes at 5 minutes and does not interfere with the analytes of interest. This new method is compatible with four existing methods (OSHA Methods 34, 36, 40 and 41), while providing improved peak shape and faster analysis time.