AN1070: Determination of Inositol Phosphates in Dried Distillers Grains with Solubles

Instrument Type: IC

A method to determine the level of phytate hydrolysis products in dried distiller grains with soluble (DDGS) is useful in determining phosphorus bioavailability to livestock animals. This study demonstrates the determination of 25 inositol phosphate isomers—InsP2–InsP6—in an in-house prepared reference solution with the method applied to a DDGS sample. Separation of the InsP isomers was achieved using a Dionex CarboPac PA100 column (4 × 250 mm) in <42 min with an HCl gradient, followed by a postcolumn reaction with ferric nitrate in dilute perchloric acid and then UV detection.

AN1044:Determination of Anions in Dried Distillers Grains with Solubles

Instrument Type: IC

This method provides a simple and accurate approach for determining water-soluble anions (e.g., chloride, sulfate, and phosphate) that contribute to the nutritional composition of DDGS. The separation is achieved on a Dionex IonPac AS11 column in <10 min. The method demonstrates excellent peak shape, linearity (r2 >0.999), accuracy (spiked recoveries 96–107%), precision (peak area RSDs <1), and detection limits in the low μg/L range (LODs 1.7–5.7 μg/L and LOQs 5.7–18.8 μg/L).