Determination of Fusarium Mycotoxins in Wheat, Maize and Animal Feed Using an Online TurboFlow and Orbitrap LC/MS Method

Instrument Type: LCMSMS

We describe a method using the Thermo Scientific Transcend TLX system with TurboFlow technology, which combines chromatography with high-resolution mass spectrometry, was developed and validated in house for the determination of deoxynivalenol, zearalenone, T-2 toxin, HT-2 toxin, fumonisins B1 and B2 in maize (corn), wheat and animal feed. Linearity range, limit of detection (LOD), limit of quantification (LOQ), recovery, repeatability, intermediate precision and accuracy were established by analysis using certified reference materials and the performance of successful proficiency testing.