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AN253: HPAE-PAD Determination of Infant Formula Sialic Acid

Instrument Type: IC

Sialic acids in infant formulas are accurately determined by HPAE-PAD using the Dionex CarboPac PA20 column following acid hydrolysis and maltodextrin removal using one of two sample-preparation methods. HPAE-PAD provides reliable determination of sialic acids in acid hydrolyzed infant formula samples without sample derivatization. This method may be used to quantify sialic acids in formulas that have been enriched with sialic acids.