Fast analysis of sugar phosphates by HPLC-CAD

Instrument Type: HPLC-CAD

The Thermo Scientific UltiMate 3000RS system is applied for the analysis of sugar phosphates. The separation is performed using a Thermo Scientific GlycanPac AXH-1 1.9µm column, with charged aerosol detection. The Thermo Scientific GlycanPac AXH-1 is a column designed to classify anionic carbohydrates by charge and resolve them by HILIC interactions. For this group of simple sugar phosphates, an isocratic analysis is sufficient using a volatile mobile phase that is compatible with charged aerosol detection or mass spectrometry.