AN1070: Determination of Inositol Phosphates in Dried Distillers Grains with Solubles

Instrument Type: IC

A method to determine the level of phytate hydrolysis products in dried distiller grains with soluble (DDGS) is useful in determining phosphorus bioavailability to livestock animals. This study demonstrates the determination of 25 inositol phosphate isomers—InsP2–InsP6—in an in-house prepared reference solution with the method applied to a DDGS sample. Separation of the InsP isomers was achieved using a Dionex CarboPac PA100 column (4 × 250 mm) in <42 min with an HCl gradient, followed by a postcolumn reaction with ferric nitrate in dilute perchloric acid and then UV detection.