Determination of Hoodigosides by UHPLC-CAD on Vanquish

Instrument Type: Unspecified

This application highlights the complimentary sensitivity of UHPLC with charged aerosol and diode array detection for determination of hoodigosides in plant extracts and dietary supplements. Eight oxypregnane steroidal glycosides isolated from dried plant material are separated from one another and from excipients such as stearic acid within 20 min on a Thermo Scientific™ Vanquish ™ UHPLC system paired with a Thermo Scientific™ Vanquish™ C18 analytical column and detected by both charged aerosol and UV/Vis diode array detectors.